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Product NameRat IL-17A ELISA Kit
Customs NameRat IL-17A ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Sample Volume10 μl
Standard Curve Range3.13 – 200 pg/ml
Spike Recovery Range94 % – 119 %
Mean Spike Recovery1.03
CV of Intra plate3.9 % – 7.1 %
CV of Inter plate3.0 % – 3.9 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-17A
Rat IL-17A Standard, lyophilized
IL-17A Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-17A. The Rat IL-17A ELISA is for research use only. Not for use in therapeutic procedures.
IL-17, also known as IL-17A and CTLA-8, is a homodimeric cytokine of about 32 kDa. IL-17 is a cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation, similar to IFN-γ. IL-17 is produced by T-helper cells and is induced by IL-23 which results in destructive tissue damage in delayed-type reactions. IL-17 as a family functions as a proinflammatory cytokine that responds to the invasion of the immune system by extracellular pathogens and induces destruction of the pathogen’s cellular matrix. IL-17 acts synergistically with TNF and IL-1.
IL-17 family has been linked to many immune / autoimmune related diseases including rheumatoid arthritis, asthma, lupus, allograft rejection, anti-tumour immunity and recently Psoriasis.Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease, allograft rejection, psoriasis, cancer and multiple sclerosis.
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